Assigning function to yeast proteins by integration of technologies.
http://yeastrc.org/unknown_orfs url MI:0614
tdavis@u.washington.edu contact-email MI:0634
Host Organism{names=Names{shortLabel='yeasx', fullName='Saccharomyces cerevisiae (Baker's yeast)', aliases=[Alias{value='Baker's yeast', type='synonym', typeAc='MI:1041'}]}, cellType=null, compartment=null, tissue=null, ncbiTaxId=4932}
Hazbun TR., Malmstrom L., Anderson S., Graczyk BJ., Fox B., Riffle M., Sundin BA., Aranda JD., McDonald WH., Chiu CH., Snydsman BE., Bradley P., Muller EG., Fields S., Baker D., Yates JR., Davis TN. author-list MI:0636
The following dataset contains a number of proteins which apparently make a high number of interactions and users may wish to filter them out as 'sticky' proteins. Specifically, the following proteins make more than 50 interactions each - P38074 (YBR034C), P38316 (YBR217W), P39081 (YDR228C), Q05583 (YDR267C), P53215 (YGR024C), P14693 (YHR083W), P38829 (YHR122W), Q3E7Z4 (YIL046W), P47088 (YJR013W), Q06411 (YLR424W), Q02159 (YMR022W), P53919 (YNL124W) and Q08273 (YOL133W). This dataset contains data not described in the original publication but which is available at the author's website. caution MI:0618
Proteins were mapped to UniProtKB entries using the ordered-locus name given by the authors. Some ordered-locus names correspond to a common UniProtKB entry as ORFs had been merged. Some ORFs had been deleted from SGD site thus they were not included. The locus history can be found on the SGD web site (http://www.yeastgenome.org/). When the same interaction was found in different screens, the interaction with the highest number of hits was added to the list. data-processing MI:0633
100 uncharacterized but essential ORFs of the yeast S. cerevisaie were fused to Gal4 DNA binding domain and tested against a genome-wide two hybrid array. exp-modification MI:0627
number of hits confidence-mapping MI:0622

Chromosome transmission fidelity protein 1

Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial

Leucine-rich repeat-containing protein SOG2

Putative uncharacterized protein YNR025C

Required for respiratory growth protein 1, mitochondrial

Translation initiation factor four A-like protein 1

Putative transposon Ty5-1 protein YCL075W

Succinate dehydrogenase assembly factor 3, mitochondrial

Increased recombination centers protein 15

Mitochondrial import inner membrane translocase subunit TIM22

DNA-directed RNA polymerase I 190 kDa polypeptide
DNA-directed RNA polymerase I subunit RPA190
DNA-directed RNA polymerase I largest subunit

Mitochondrial fusion and transport protein UGO1

Inosine-5'-monophosphate dehydrogenase 2

Mitochondrial import inner membrane translocase subunit TIM50

Endosomal/prevacuolar sodium/hydrogen exchanger

Putative regulator of rDNA transcription protein 16

Putative uncharacterized protein YLL020C

Putative uncharacterized protein YPR146C

Putative uncharacterized protein YCL068C

Serine/threonine-protein phosphatase 1 regulatory subunit PIG1

DNA replication fork-blocking protein FOB1

Succinate dehydrogenase assembly factor 4, mitochondrial

General negative regulator of transcription subunit 2

Sulfite reductase [NADPH] flavoprotein component

Putative uncharacterized protein YLR217W

U3 small nucleolar RNA-associated protein 8

Mitochondrial intermembrane space cysteine motif-containing protein MIX17
Mitochondrial intermembrane space CX(n)C motif protein of 17 kDa

Uncharacterized membrane protein YMR253C

Ubiquitin domain-containing protein DSK2

Arginine metabolism regulation protein III

DNA-(apurinic or apyrimidinic site) lyase 1

Glycerol uptake/efflux facilitator protein

Putative uncharacterized protein YKL066W

Deoxyribodipyrimidine photo-lyase, mitochondrial

Putative tyrosine-protein phosphatase OCA6

RNA polymerase II transcriptional coactivator SUB1

Peptidyl-prolyl cis-trans isomerase CPR6

Putative uncharacterized protein YDL071C

Probable 26S proteasome regulatory subunit p27

Putative uncharacterized protein YJR120W

Nuclear distribution protein nudE-like 1
Nuclear distribution protein nudE homolog 1

Vacuolar membrane-associated protein IML1

Pyridoxal 5'-phosphate synthase glutaminase subunit
Probable pyridoxal 5'-phosphate synthase subunit SNO3

Mitochondrial large ribosomal subunit protein mL43
54S ribosomal protein L51, mitochondrial

Putative uncharacterized protein YOR392W

Putative uncharacterized protein YMR052C-A

Peptidyl-prolyl cis-trans isomerase ESS1

Mediator of RNA polymerase II transcription subunit 6

Ubiquitin carboxyl-terminal hydrolase 10

DNA replication complex GINS protein PSF3

Chromatin structure-remodeling complex subunit SFH1

Probable dolichyl-phosphate-mannose--protein mannosyltransferase 7

Cyclin-dependent kinases regulatory subunit

Putative uncharacterized protein YKL147C

Target of rapamycin complex 1 subunit TCO89

Putative uncharacterized protein YMR086C-A

Guanine nucleotide exchange factor SDC25

Putative uncharacterized protein YIR014W

Signal recognition particle subunit SRP68

Structural maintenance of chromosomes protein 2

Regulator of Ty1 transposition protein 107

ABC transporter ATP-binding protein ARB1

Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51

Grand meiotic recombination cluster protein 1

54S ribosomal protein L37, mitochondrial

Translation machinery-associated protein 46

Uncharacterized mitochondrial membrane protein FMP10

DNA polymerase alpha catalytic subunit A

Probable target of rapamycin complex 2 subunit BIT2

Iron-sulfur clusters transporter ATM1, mitochondrial

Oxidant-induced cell-cycle arrest protein 6

3-hydroxy-3-methylglutaryl-coenzyme A reductase 2

DNA polymerase zeta processivity subunit

DNA replication complex GINS protein SLD5

Ubiquitin-specific-processing protease 2

Putative uncharacterized protein YPR092W

Non-structural maintenance of chromosome element 3

Nicotinamide mononucleotide adenylyltransferase

Putative nucleotide-sugar transporter YMD8

Uncharacterized protein YLR346C, mitochondrial

Mitochondrial 13 kDa outer membrane protein

Putative uncharacterized protein YLR279W

Inositol phosphosphingolipids phospholipase C

Glycolytic genes transcriptional activator GCR1

Anaphase-promoting complex subunit CDC23

37S ribosomal protein S18, mitochondrial

60S ribosome subunit biogenesis protein NOP8

Clathrin assembly protein complex 1 mu-1-I medium chain

Putative uncharacterized protein YLR366W

COX3 mRNA-specific translational activator PET494

Sorting assembly machinery 50 kDa subunit

Non-structural maintenance of chromosome element 4

Presequence translocated-associated motor subunit PAM17, mitochondrial

Uncharacterized PH domain-containing protein YPR091C

Probable ferric reductase transmembrane component 8

Mediator of RNA polymerase II transcription subunit 21

Putative uncharacterized protein YGL102C

E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5

Tyrosine-protein phosphatase-like protein OCA2

Enhanced spontaneous mutability protein 3

RNA polymerase II subunit A C-terminal domain phosphatase

Hypersensitivity to hygromycin-B protein 1

Down syndrome candidate region 1-like protein

Putative uncharacterized protein YOR282W

37S ribosomal protein MRP1, mitochondrial
Mitochondrial small ribosomal subunit protein mS43

Spindle pole body assembly component MPS3

Serine/threonine-protein phosphatase PP-Z1

Transcriptional regulatory protein SAP30

Mitochondrial small ribosomal subunit protein uS11m

DNA topoisomerase 2-associated protein PAT1

Upstream activation factor subunit UAF30

Putative uncharacterized protein YKR033C

Mitochondrial organization of gene expression protein 5

Uncharacterized membrane protein YPL257W

Endoplasmic reticulum vesicle protein 25

S-methylmethionine:homocysteine methyltransferase 1

Heat shock protein Hsp90 constitutive isoform

Regulator of V-ATPase in vacuolar membrane protein 2

Putative uncharacterized protein YJR087W

U3 small nucleolar RNA-associated protein 19

Conserved oligomeric Golgi complex subunit 6

Mediator of RNA polymerase II transcription subunit 2

Serine/threonine-protein kinase/endoribonuclease IRE1

Uncharacterized ORAOV1 family protein YNL260C

N-terminal acetyltransferase B complex catalytic subunit NAT3
NatB Nalpha terminal acetyltransferase 3

Vacuolar protein-sorting-associated protein 24

Mitochondrial small ribosomal subunit protein uS8m

Constitutive centromere-associated network protein NKP1

Chromatin structure-remodeling complex protein ARP7

Putative pterin-4-alpha-carbinolamine dehydratase
4-alpha-hydroxy-tetrahydropterin dehydratase

54S ribosomal protein L22, mitochondrial
Mitochondrial large ribosomal subunit protein uL22m

6-phosphogluconate dehydrogenase, decarboxylating 2

Phosphopantothenoylcysteine decarboxylase subunit SIS2

Putative uncharacterized protein YML009W-B

ATP-binding cassette protein involved in ribosome biogenesis 1

ADA histone acetyltransferase complex component 2

Ran-specific GTPase-activating protein 1

Putative uncharacterized protein YCL042W

Required for respiratory growth protein 8, mitochondrial
Non-structural maintenance of chromosomes element 1
Histone-lysine N-methyltransferase, H3 lysine-4 specific
Putative uncharacterized protein YDR355C
Hydroxymethylbilane hydrolyase [cyclizing]
Cytochrome oxidase assembly protein SHY1
Peroxisomal acyl-coenzyme A thioester hydrolase 1
Repressor/activator site-binding protein
13 kDa ribonucleoprotein-associated protein
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Putative uncharacterized protein YPR076W
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Aspartate-beta-semialdehyde dehydrogenase
CCA tRNA nucleotidyltransferase, mitochondrial
Peptidyl-prolyl cis-trans isomerase C, mitochondrial
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Asparagine-linked glycosylation protein 2
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
Regulation of Ty1 transposition protein 104
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Oxidant-induced cell-cycle arrest protein 5
Trivalent metalloid-sensitive Cuz1-related protein 1
Putative uncharacterized protein YBR134W
Disrupter of telomere silencing protein 4
Ubiquitin-specific-processing protease 10
Resistance to o-dinitrobenzene protein 1
Complex proteins associated with SET1 protein SWD3
Cytochrome c oxidase subunit 6, mitochondrial
25S rRNA (adenine(645)-N(1))-methyltransferase
Multicopy suppressor of defective G-protein 1
Vacuolar protein sorting-associated protein 44
Endosomal/prevacuolar Na(+)/H(+) exchanger
Isopentenyl-diphosphate: tRNA isopentenyltransferase
Target of rapamycin complex 2 subunit BIT61
External NADH-ubiquinone oxidoreductase 1, mitochondrial
Chitin-UDP acetyl-glucosaminyl transferase 3
Glutamyl-gamma-semialdehyde dehydrogenase
Decapping activator and translational repressor PAT1
Topoisomerase II-associated protein PAT1
Serine/threonine-protein phosphatase PP-Z2
General negative regulator of transcription subunit 1
Altered inheritance of mitochondria protein 12
Chromatin structure-remodeling complex subunit RSC7
Remodel the structure of chromatin complex subunit 7
Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit
Required for meiotic nuclear division protein 7
Guided entry of tail-anchored proteins 2
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61
General control non-derepressible protein 14
tRNA(m1A58)-methyltransferase subunit TRM61
DNA polymerase alpha:primase complex p180 subunit
Upstream activation factor 30 KDa subunit
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
Putative uncharacterized protein YOR318C
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Ubiquinone biosynthesis methyltransferase COQ5
2-hexaprenyl-6-methoxy-1,4-benzoquinol methyltransferase
Clathrin assembly protein large beta-1 chain
Clathrin assembly protein complex 1 beta-1 large chain
RNA-binding suppressor of PAS kinase protein 1
Hexaprenyl pyrophosphate synthase, mitochondrial
Uncharacterized membrane protein YNL320W
pH-response transcription factor pacC/RIM101
Disrupter of telomere silencing protein 5
Probable transcriptional regulatory protein HAH1
Transcription initiation factor TFIID subunit 9
Late endosome and vacuole interface protein 7
Mitochondrial respiratory function protein 1
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
Increased recombination centers protein 9
Small nuclear ribonucleoprotein-associated protein 1
N-terminal acetyltransferase C complex catalytic subunit MAK3
L-A virus GAG protein N-acetyltransferase subunit MAK3
General transcriptional corepressor TUP1
Putative uncharacterized protein YPL182C
23 kDa translation machinery-associated protein
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI12
ATP-dependent 6-phosphofructokinase subunit alpha
Conserved oligomeric Golgi complex subunit 3
Oxidant-induced cell-cycle arrest protein 2
Putative uncharacterized protein YLR415C, mitochondrial
Suppresses postrecruitment functions protein 1
Biogenesis of lysosome-related organelles complex 1 subunit KXD1
Signal recognition particle 68 kDa protein homolog
Mitochondrial organization of gene expression protein 7
Found in mitochondrial proteome protein 21
ERAD-associated E3 ubiquitin-protein ligase component HRD3
Cardiolipin-specific deacylase 1, mitochondrial
DNA-(apurinic or apyrimidinic site) lyase 2
Suppressor of defective silencing protein 3
Glucose repression regulatory protein TUP1
Vacuolar protein-sorting-associated protein 31
BCK1-like resistance to osmotic shock protein 1
Nitrogen permease inactivating protein 3
Mitochondrial large ribosomal subunit protein mL54
Genes involved in microtubule biogenesis protein 2
E3 ubiquitin-protein ligase substrate receptor MMS22
Cytoplasm to vacuole targeting protein 7
E3 ubiquitin ligase complex SCF subunit CDC34
Guided entry of tail-anchored proteins 3
Meiosis and centromere regulatory kinase
GTPase EF-hand protein of mitochondria 1
Putative uncharacterized protein YOL114C
Multicatalytic endopeptidase complex subunit PRE2
3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
Synthetic lethal of unknown function protein 5
Arginine metabolism regulation protein II
Found in mitochondrial proteome protein 32
Endoplasmic reticulum-to-nucleus signaling 1
Putative uncharacterized protein YLR156W/YLR157W-D/YLR159W/YLR161W
Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1
Protein arginine N-methyltransferase HSL7
Type II protein arginine N-methyltransferase
A-agglutinin cell wall attachment subunit
Thioredoxin-dependent peroxide reductase
Putative aryl-alcohol dehydrogenase AAD15
Altered inheritance rate of mitochondria protein 45
Probable electron transfer flavoprotein subunit alpha, mitochondrial
Replication protein A 69 kDa DNA-binding subunit
Peroxisomal long-chain acyl-CoA thioesterase 1
Synthetically lethal with MCM10 protein 2
Methyl methanesulfonate-sensitivity protein 22
Vacuolar protein-sorting-associated protein 32
High osmolarity glycerol response protein 1